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ECOSTM Competent Cells | 
| Chang, C. N., W. C. Huang, C. C. Chang, W. S. Tzeng, T. W. Chen, Y. S. Liu and Y.T. Shyu.2006 Tufa microbial diversity revealed by 16S rRNA cloning in Taroko National Park, Taiwan. Soil Biology and Biochemistry 38(2): 342-348.
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| Chen D. Y., H. F. NI, J. H. Yen, Y. H. Cheng and T. T. Tsay. 2005 Differentiation of the Xiphinema americanum-group nematodes X. brevicollum, X. incognitum, X. diffusum and X. oxycaudatum in Taiwanby morphometrics and nuclear ribosomal DNA sequences. Nematology 7(5): 713-725.
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| Chen, Y. H., Y. C. Kam, and H.T. Yu. 2005 Isolation and characterization of polymorphic microsatellite loci for parentage analysis in a rhacophorid tree frog (Chirixalus eiffingeri) with unusual parental care. Molecular Ecology 5: 430-432.
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| Huang K. J., C. C. Ku and I.R. Lehman. 2006 Endonuclease G: A role for the enzyme in recombination and cellular proliferation PNAS. 103(24): 8995-9000.
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| Hwang, S. H., C. H. Cha, M.D., Y. L. Kim, O. J. Kwon and H. B. Oh. 2006 Performance Evaluation of Real-Q HBV Quantification Kit for HBV DNA by Real-Time PCR. Korean J Lab Med. 26(6): 442-448.
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| Kang, H., H. S. Yun, J. K. Lee and K. Kwack. 2008 Identification of a novel HLA-G allele, HLA-G*010113, in a Korean individual. Tissue Antigens 71(5): 492-494.
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| Liao, J. Y., C. H. Chung, T. K. Lin, C. A. Chang and T. C. Deng1. 2007 Identification of Squash leaf curl Philippines virus on Benincasa hispida in Taiwan. Plant Pathology Bulletin 16:11-18.
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| Lim, C. T. and J. C. H. Goh. 2008 13th International Conference on Biomedical Engineering IFMBE Proceedings 23:1393-1396.
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| Lim, C. T. and J. C. H. Goh. 2009 99mTc(I)-tricarbonyl Labeled Histidine-tagged Annexin V for Apoptosis Imaging. IFMBE Proceedings 23: 1393-1396.
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| Liu, S. Y. V., C. F. Dai, T. Y. Fan and H. T. Yu. 2005 Cloning and Characterization of Microsatellite Loci in a Gorgonian Coral, Junceella juncea (Anthozoa; Octocorallia; Ellisellidae) and Its Application in Clonal Genotyping. Marine Biotechnology 7(1): 26-32.
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| Liu, Y. L., L. M. Huang, W. P. Lin, C. C. Tsai, T. S. Lin, Y. H. Hu, H. S. Chen, J. M. Han, H. J. Wang, Y. T. Liu. 2006 Secretion of biologically active human epidermal growth factor from Escherichia coli using Yersinia pestis Caf1 signal peptide. J. Microbiol Immunol Infect. 39(5):366-71.
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| Ng, C.C. and Y.T. Shyu, 2005 Archaeal Community Revealed by 16s rRNA and Fluorescence in situ Hybridization in a Sulphuric Hydrothermal Hot Spring, Northern Taiwan. World J. of Microbiology and Biotechnology 21(6-7): 933-939.
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| Pan, Y. W., H. H. Chou, E. M.You and H. T. Yu. 2004 Isolation and characterization of 23 polymorphic microsatellite markers for diversity and stock analysis in tiger shrimp (Penaeus monodon). Molecular Ecology Notes 4(3): 345-7.
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| Park, S. L., B.H. Lee and D. J. Kim 2009 Identification of Proteins That Interact with Podocin Using the Yeast 2-Hybrid System. Yonsei Med J. 50: 273-279.
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| Shih, F. L., Y. P. Cheng, S. Y. Hwang and T. P. Lin. 2006 Partial Concordance between Nuclear and Organelle DNA in Revealing the Genetic Divergence among Quercus glauca (Fagaceae) Populations in Taiwan. International Journal of Plant Sciences 167(4): 863-872.
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| Shkolnik, k., S. Ben-Dor, D. Galiani, A. Hourvitz and N. Dekel. 2008 Molecular characterization and bioinformatics analysis of Ncoa7B, a novel ovulation-associated and reproduction system-specific Ncoa7 isoform. Reproduction 135(3):321-33.
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| Steain, M. C., B. Wang and N. K. Saksena. 2008 The possible contribution of HIV-1-induced syncytia to the generation of intersubtype in vitro. AIDS 22(9): 1009-1017.
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| Wu, C. F., M. W. Yu, C. L. Lin, C. J. Liu, W. L. Shih, K. S. Tsai and C. J. Chen. 2008 Long-term tracking of hepatitis B viral load and the relationship with risk for hepatocellular carcinoma in men. Carcinogenesis 29(1): 106-112.
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| Wu, H. C., H. J. Su and J. M. Hu. 2007 The identification of A-, B-, C-, and E-class MADS-box genes and implications for perianth evolution in the basal eudicot Trochodendron aralioides (Trochodendraceae). International Journal of Plant Sciences 168: 775-799.
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| Yamada, K., T. Terahara,S. Kurata, T. Yokomaku,S. Tsuneda and S. Harayama. 2008 Retrieval of entire genes from environmental DNA by inverse PCR with re-amplification of target genes using primers containing locked nucleic acids. Environ Microbiol 10(4): 978-987.
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| Zhou, Z., L. Hong., X. Shen., X. Rao., X. Jin., G. Lu., L. Li., E. Xiong., W. Li and J. Zhang. 2008 Detection of Nanobacteria Infection in Type III Prostatitis. Urology 71(6): 1091-1095.
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| Citations | | |
T&ATM Cloning System |
| Chang, L. Y., B. L. Chiang, C. L. Kao, M. H. Wu, P. J. Chen and L. M. Huang. 2006 Lack of Association between Infection with a Novel Human Coronavirus ( Hcov), Hcov-Nh, and Kawasaki Disease in Taiwan. Journal of Infectious Diseases 193(2): 283-286.
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| Chen, D. Y., H. F. Ni, J. H.Yen, Y. H. Chen and T. T. Tsay. 2005 Differentiation of the Xiphinema americanum-group nematodes X. brevicollum, X. incognitum, X. diffusum and X. oxycaudatum in Taiwan by morphometrics and nuclear ribosomal DNA sequences. Nematology 7(5): 713-725.
| | Chu, C. C., W. C. Lee, W. Y. Guo, S. M. Pan, L. J. Chen, H. M. Li and T. L. Jinn. 2005 A Copper Chaperone for Superoxide Dismutase That Confers Three Types of Copper/Zinc Superoxide Dismutase Activity in Arabidopsis. Plant Physiol 139(1): 425-436.
| | Chuang, M. H., M. S. Wu, W. L. Lo, J. T. Lin, C. H. Wong and S. H. Chiou. 2006 The antioxidant protein alkylhydroperoxide reductase of Helicobacter pylori switches from a peroxide reductase to a molecular chaperone function. PNAS 103(8): 2552-2557.
| | Hsieh, C. Y., Z. B. Wu, M. C. Tung and S. S. Tsai. 2007 PCR and in situ hybridization for the detection and localization of a new pathogen Francisella-like bacterium (FLB) in ornamental cichlids. Diseases of aquatic organisms 75(1): 29-36.
| | Hsieh, C. Y., Z. B. Wu, M. C. Tung, C. Tu, S. P. Lo, T. C. Chang, C. D. Chang, S. C. Chen, Y. C. Hsieh and S. S. Tsai. 2006 In situ hybridization and RT-PCR detection of Macrobrachium rosenbergii nodavirus in giant freshwater prawn, Macrobrachium rosenbergii (de Man), in Taiwan. Journal of Fish Diseases 29(11): 665-671.
| | Hsieh, W.J., Y. F. Chang, C. S. Chen and M. J. Pan. 2005 Omp52 is a growth-phase-regulated outer membrane protein of Leptospira santarosai serovar Shermani. FEMS microbiology letters 243(2): 339-345.
| | Hsu, C.N. and C. H. Wang. 2006 Sequence comparison between two quasi strains of H6N1 with different pathogenicity from a single parental isolate. The Journal of Microbiology, Immunology and Infection 39(4): 292-6.
| | Hsu, S. C. and C. J. Huang. 2007 Changes in liver PPAR α mRNA expression in response to two levels of high-safflower-oil diets correlate with changes in adiposity and serum leptin in rats and mice. The Journal of nutritional biochemistry 18(2): 86-96.
| | Hsu, W. B., J. H. Wang, P. C. Chen, Y. S. Lu and J. H. Chen. 2007 Detecting low concentrations of Shigella sonnei in environmental water samples by PCR. FEMS Microbiology Letters 270(2): 291-298.
| | Huang, M. T., H. S. Tsao and H. T. Yu. 2003 Isolation and cross-species amplification of microsatellite loci in the freshwater minnow Zacco pachycephalus (Teleostei: Cyprinidae) for diversity and conservation genetic analysis. Molecular Ecology Notes 3(4): 567-569.
| | Huang,Y. C., Y. H. Lin, C. H. Shih, C. L. Shih, T. Chang and C. J. Chen. 2006 Purification, crystallization and preliminary X-ray crystallographic analysis of rice lectin from Oryza sativa. Acta Crystallographica Section F: Structural Biology Crystallographica Communication 62(2): 94-96.
| | Kung, S. S., Y. C. Chuang, C. H. Chen and C. C. Chien. 2007 Isolation of polyhydroxyalkanoates-producing bacteria using a combination of phenotypic and genotypic approach. Letters in Applied Microbiology 44(4): 364-371.
| | Liu, S. Y., H. T. Yu and C. F. Dai. 2007 Eight microsatellite loci in Clark's anemonefish, Amphiprion clarkii. Molecular Ecology Notes 7(6): 1169-1171.
| | Moon, G. S., Y. R. Pyun, M. S. Park, G. E. Ji and W. J. Kim. 2005 Secretion of Recombinant Pediocin PA-1 by Bifidobacterium longum, Using the Signal Sequence for Bifidobacterialα-Amylase. Applied and Environmental Microbiology 71(9): 5630-5632.
| | Tsai, C. L., H. P. Li, Y. J. Lu, C. Hsueh, Y. Liang, C. L. Chen, S. W. Tsao, K. P. Tse, J. S. Yu and Y. S. Chang. 2006 Activation of DNA Methyltransferase 1 by EBV LMP1 Involves c-Jun NH2-Terminal Kinase Signaling. Cancer Research 66(24): 11668-11676.
| | Wang, J. M., C. Y. Ko, L. C. Chen, W. L. Wang and W. C. Chang. 2006 Functional role of NF-IL6β and its sumoylation and acetylation modifications in promoter activation of cyclooxygenase 2 gene. Nucleic Acids Research 34(1): 217-231.
| | Wang, T. C. and M. Chao. 2005 RNA Recombination of Hepatitis Delta Virus in Natural Mixed-Genotype Infection and Transfected Cultured Cells. American Society for Microbiology 79(4): 2221-2229.
| | Weng, M. W., J. C. Lai, C. P. Hsu, K. Y. Yu, C. Y. Chen, T. S. Lin, W. W. Lai, H. Lee and J. L. Ko. 2005 Alternative splicing of MDM2 mRNA in lung carcinomas and lung cell lines. Environmental and Molecular Mutagenesis 46(1): 1-11.
| | Wu, H.L., L.R. Huang, C. C. Huang, H. L. Lai, C. J. Liu, Y. T. Huang, Y. W. Hsu, C. Y. Lu, D. S. Chen and P. J. Chen. 2005 RNA interference-mediated control of hepatitis B virus and emergence of resistant mutant. Gastroenterology 128(3): 708-16.
| | Wu, M. L., T. P. Lin, M. Y. Lin, Y. P. Cheng and S. Y. Hwang. 2007 Divergent Evolution of the Chloroplast Small Heat Shock Protein Gene in the Genera Rhododendron (Ericaceae) and Machilus (Lauraceae). Annals of Botany 99(3): 461-475.
| | Wu, S.Y., C. H. Hung, C. N. Lin, H. W. Chen, A. S. Lee and J. S. Chang. 2006 Fermentative hydrogen production and bacterial community structure in high-rate anaerobic bioreactors containing silicone-immobilized and self-flocculated sludge. Biotechnology and Bioengineering 93(5): 934-46.
| | Wu, Y.H., J. H. Tsai-Chang, Y. W. Cheng, T. C. Wu, C. Y. Chen and H. Lee. 2007 Xeroderma pigmentosum group C gene expression is predominantly regulated by promoter hypermethylation and contributes to p53 mutation in lung cancers. Oncogene 26(33): 1-13.
| | Young, K. C., C. T. Huang, S. L. Lin and W. C. Hsiao. 2006 Two-Phased Alteration and Clinical Relevance of MTA1 and MTA1s Gene Expression: A Quantitative PCR Analysis in Breast Cancer Patients. Journal of Cancer Molecules 2(2): 79-84.
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| Citations | | |
DNA Polymerase
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| Chen, D. Y., H. F. Ni, J. H.Yen, Y. H. Chen and T. T. Tsay. 2005 Differentiation of the Xiphinema americanum-group nematodes X. brevicollum, X. incognitum, X. diffusum and X. oxycaudatum in Taiwan by morphometrics and nuclear ribosomal DNA sequences. Nematology 7(5): 713-725.
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| Chou, C. M., C. J. Huang, C. M. Shih, Y. P. Chen, T. P. Liu and C. T. Chen. 2005 Identification of Three Mutations in the Cu,Zn-Superoxide Dismutase (Cu,Zn-SOD) Gene with Familial Amyotrophic Lateral Sclerosis: Transduction of Human Cu,Zn-SOD into PC12 Cells by HIV-1 TAT Protein Basic Domain. Annals of the New York Academy of Science 1042: 303-313.
| | Hsu, Y. H., L. S. Wang, L. K. Chen and H. H. Yang. 2005 Rabies Virus Infection: Report of an Autopsy Case with Comprehensive Pathologic, Immunofluorescent, Immunohistochemical and Molecular Studies. Tzu Chi Medical Journal 17(4): 219-225.
| | Huang, F. C., C. S. Liao and G. B. Lee. 2006 An integrated microfluidic chip for DNA/RNA amplification, electrophoresis separation and on-line optical detection. Electrophoresis 27(16): 3297-3305.
| | Huang, F.C., Y. F. Chen and G. B. Lee. 2007 CE chips fabricated by injection molding and polyethylene/thermoplastic elastomer film packaging methods. Electrophoresis 28(7): 1130-1137.
| | Huang, Y. H., C. M. Shih, C. J. Huang, C. M. Lin, C. M. Chou, M. L. Tsai, T. P. Liu, J. F. Chiu and C. T. Chen. 2006 Effects of cadmium on structure and enzymatic activity of Cu,Zn-SOD and oxidative status in neural cells. Journal of Cellular Biochemistry 98(3): 577-589.
| | Hung, L. M., W. Wei, Y. J. Hsueh, W. K. Chu and F. C. Wei. 2004 Ischemic Preconditioning Ameliorates Microcirculatory Disturbance through Downregulation of TNF-Alpha Production in a Rat Cremaster Muscle Model. Journal of Biomedical Science 11(6): 773-780.
| | Wei, W., F. C. Wei and L. M. Hung. 2005 Diazoxide ameliorates microcirculatory disturbances through PKC-dependent Pathway in I/R-injured rat cremaster muscles. Journal of Biomedical Science 12(3): 521-529.
| | Yeh, S. H., S. C. Mao, H. C. Lin and P. W. Gean. 2006 Synaptic Expression of Glutamate Receptor after Encoding of Fear Memory in the Rat Amygdala. Molecular Pharmacology 69(1): 299-308.
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| Citations | | | DNA Markers | | Hsieh ,T. M., C. H. Luo, G. B. Lee, C. S. Liao and F. C. Huang. 2006 A Micromachined Low-power-consumption Portable PCR System. Journal of Medical and Biological Engineering 26(1): 43-49.
| | Hsieh, T. M., C. H. Luo, G. B. Lee, F. C. Huang and C. S. Liao. 2006 A new control scheme for micromachine-based PCR chips on glass substrates. Asia-Pacific Conference of Transducers and Micro-Nano Technology.
| | Liao, C. S., G.. B. Lee, H. S. Liu, T. M. Hsieh and C. H. Luo. 2005 Miniature RT–PCR system for diagnosis of RNA-based viruses. Nucleic Acids Research 33(18): 156.
| | Wang, C. H., Y. Y. Chen, C. S. Liao, T. M. Hsieh, C. H. Luo, J. J. Wu, H. H. Lee and G. B. Lee. 2007 Circulating polymerase chain reaction chips utilizing multiple-membrane activation. Journal of Micromechanics and Microengineering 17(2): 367-375.
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| Citations | | |
YLEX Expression Kits
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| High-throughput screeningin Yarrowia lipolytic
Bordes, F., F. Fudalej, V. Dossat, J. M. Nicaud and A. Marty. 2007 A new recombinant protein expression system for high-throughput screening in the yeast Yarrowia lipolytica. Journal of Microbiological Methods 70(3): 493-502.
| | One-step transformation of Yarrowia lipolytica
Chen, D. C., J. M. Beckerich and C. Gaillardin. 1997 One-step transformation of the dimorphic yeast Yarrowia lipolytica. Applied Microbiology Biotechnology 48(2): 232-235.
| | | | Crystallization of a recombinant maize cytokinin oxidase produced inYarrowia lipolytica.
Kopecny, D., P. Briozzo, N. Joly, N. Houba-Hérin, C. Madzak, C. Pethe and M. Laloue. 2004 Purification, crystallization and preliminary X-ray diffraction study of a recombinant cytokinin oxidase from Zea mays. Acta Crystallographica Section D 60: 1500–1501. | | Expression of a maize cytokinin oxidase/dehydrogenase Kopecny, D., C. Pethe, M. Sebela, N. Houba-Herin, C. Madzak, A. Majira and M. Laloue. 2005 High-level expression and characterization of Zea mays cytokinin oxidase/dehydrogenase in Yarrowia lipolytica. Biochimie 87(11): 1011-1022.
| | Upstream regulating regions of the XPR2 promoter Madzak, C., S. Blanchin-Roland, R. R. C. Otero and C. Gaillardin. 1999 Functional analysis of upstream regulating regions from the Yarrowia lipolytica XPR2 promoter. Microbiology 145(1): 75-87.
| | Strong hybrid promoters for quasi-constitutive expression
Madzak, C., B. Treton and S. Blanchin-Roland. 2000 Strong hybrid promoters and integrative expression/secretion vectors for quasi-constitutive expression of heterologous proteins in the yeast Yarrowia lipolytica. Journal of Molecular Microbiology and Biotechnology 2(2): 207-216.
| | A review on heterologous production in Yarrowia lipolytica / comparison of several yeast expression systems.
Madzak, C., C. Gaillardin and J. M. Beckerich. 2004 Heterologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica: a review. Journal of Biotechnology 109(1-2): 63-81.
| | | | Engineering of a fungal laccase in Yarrowia lipolytica
Madzak, C., M. C. Mimmi, E. Caminade, A. Brault, S. Baumberger, P. Briozzo, C. Mougin and C. Jolivait. 2006 Shifting the optimal pH of activity for a laccase from the fungus Trametes versicolor by structure-based mutagenesis. Protein Engineering, Design & Selection 19(2): 77-84.
| | A review on Yarrowia lipolytica
Nicaud, J. M., C. Madzak, P. V. D. Broek, C. Gysler, P. Duboc, P. Niederberger and C. Gaillardin. 2002 Protein expression and secretion in the yeast Yarrowia lipolytica. FEMS Yeast Research 2(3): 371-379.
| | Surface display of recombinant proteins inYarrowia lipolytica.
Yue, L., Z. Chi, L. Wang, J. Liu, C. Madzak, J. Li and X. Wnag. 2008 Construction of a new plasmid for surface display on cells of Yarrowia lipolytica. Journal of Microbiological Methods 72(2): 116-123.
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| Citations | | |
LzipTM Ling Zhi immunomodulatory protein (LZ8) |
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| Kino, K., K. Mizumoto, T. Sone, T. Yamaji, J. Watanabe, A. Yamashita, K. Yamaoka, K. Shimizu, K. Ko and H. Tsinoo. 1990 An immunomodulating protein, Ling Zhi-8 (LZ-8) prevents insulitis in non-obese diabetic mice. Diabetologia: 33(12): 713-718.
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| Liao, C. H., Y. M. Hsiao, C. H. Lin, C. S. Yeh, C. H. Wang, C. H. Ni, C. P. Hsu and J. L. Ko. 2008 Induction of premature senescence in human lung cancer by fungal immunomodulatory protein from Ganoderma tsugae. Food and Chemical Toxicology: 46(5):1851-1859.
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| Lin, Y. L., Y. C. Liang, Y. S. Tseng, H. Y. Huang, S. Y, Chou, R. S. Hseu, C. T, Huang and B. L. Chiang. 2009 An immunomodulatory protein, Ling Zhi-8, induced activation and maturation of human monocyte-derived dendritic cells by the NF-kB and MAPK pathways. Journal of Leukocyte Biology doi: 10.1189/jlb.0708441 |
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| Murasugi, A., S. Tanaka, N. Komiyama, N. Iwata, K. Kino, H. Tsinoo and S. Sakuma. 1991 Molecular cloning of a cDNA and a gene encoding an immunomodulatory protein, Ling Zhi-8, from a fungus, Ganoderma lucidum. The Journal of Biological Chemistry : 266(4):2486-2493.
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| Tanaka, S., K. Ko, K. Kino, K. Tsuchiya, A. Yamashita, A. Murasugi, S. Sakuma and H. Tsunoo. 1989 Complete amino acid sequence of an immunomodulatory protein, ling zhi-8 (LZ-8). An immunomodulator from a fungus, Ganoderma lucidium, having similarity to immunoglobulin variable regions. The Journal of Biology Chemistry: 264(28):16372-16377.
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| Wang, P. H., J. L. Ko, H. T. Tsai, S. F. Yang, C. P. Han, L. Y. Lin and G. D. Chen. 2008 Clinical significance of matrix metalloproteinase-2 in cancer of uterine cervix: A semiquantitative study of immunoreactivities using tissue array. Gynecologic Oncology: 108(3):533-542.
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