DNA Fragment Extraction Kit
Description

The Small DNA Fragment Extraction Kit was designed to recover or concentrate DNA fragments (50-200 bp) from agarose gel, PCR, or other enzymatic reactions. Chaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer or TE. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation. Typically, recoveries are up to 90% for Gel Extraction and up to 95% for PCR Clean up. The entire procedure can be completed in 20 minutes.

The Large DNA Fragment Extraction Kit is designed to recover or concentrate DNA fragments (> 8 Kb) from agarose gel in 4 easy steps. Salts and enzymes can be effectively removed from the reaction mixture without phenol extraction. Typically, recoveries are up to 85% for Gel Extraction. The entire procedure can be completed in 45 minutes.
Features

Small DNA Fragment Extraction Kit:

  • Recovery : up to 95%
  • Operation time : within 20 minutes

 

Large DNA Fragment Extraction Kit

  • Recovery : up to 85%
  • Operation time : within 45 minutes
Applications

Fluorescent/radioactive, sequencing, PCR, restriction enzyme digestion, DNA labeling and ligation

Quality Control

The quality of the Small/Large DNA Fragment Extraction Kit is tested on a lot-to-lot basis by isolating DNA fragments of various sizes from either aqueous solutions or agarose gels. The purified DNA is checked by electrophoresis.

Specification
Store at

 

Small DNA Fragment
Extraction Kit

# FYG203-100P (100 preps)
SD Buffer 80 ml
Wash Buffer 25 ml
W1 Buffer 45 ml
Elution Buffer 6 ml
SD Column 100 pcs
2 ml Collection Tube 100 pcs
# FYG203-300P (300 preps)
SD Buffer 240 ml
Wash Buffer 50 ml
Wash Buffer 25 ml
W1 Buffer 130 ml
Elution Buffer 30 ml
SD Column 300 pcs
2 ml Collection Tube 300 pcs

 

Large DNA Fragment
Extraction Kit

# FYG204-100P (100 preps)
LD Buffer 3 ml
Wash Buffer 25 ml
Resuspension Buffer 65 ml
LD Column 100 pcs
# FYG204-300P (300 preps)
LD Buffer 10 ml
Wash Buffer 75 ml
Resuspension Buffer 30 ml
LD Column 300 pcs
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