Description

Sharp Protein Marker II is 3-color pre-stained recombinant protein standards consists of 10 proteins from 10 kDa to 315 kDa. The marker mix is pre-denatured and stabilized in loading buffer, so no additional boiling is necessary before loading onto SDS-PAGE gels.
Features

  • Sharp bands and stable colors
  • Multicolor: easy to track on gel
  • Excellent Western transfer
  • Ready-to-load: no need to boil
Applications

  • Monitoring protein migration during SDS-PAGE
  • Monitoring protein transfer onto membranes during Western blotting
  • Approximate sizing of proteins on SDS-PAGE gels
  • Acrylamide gels and Western blots
Notes

  • The ladder contains two green reference bands at ~50 kDa and 10 kDa and one orange reference band of 70 kDa.
  • Avoid multiple freeze/thaw cycles.
  • Lot-to-lot variation of the apparent molecular weight of prestained proteins is <10% depending on the molecular weight.
  • Western blotting of large (>100 kDa) proteins may need longer transfer times or higher transfer voltages for efficient transfer.
Quality Control

5 μl of Sharp Protein Marker II provide expected numbers of bands of equal intensity in SDS-PAGE (Tris-glycine buffer) and after electrotransfer onto a PVDF membrane.
Recommendation for Loading

  • Apply 5 μl/well on mini gels and 10 ~ 15 μl/well on large gels
  • For blots, apply 1 ~ 3 μl/well on mini gels and 2 ~ 6 μl/well on large gels
Results

Specification
Store at

 

# FYP005-250UL
Sharp Protein Marker II 250 μl
# FYP005-500UL
Sharp Protein Marker II 550 μl
Related Prodcuts

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